Table 1:

Changes with time of song parameters in 4 T-treated male canaries that started singing 3 days after the beginning of the treatment.

Singing behavior						statistics (F)	Effect size

MALES + T (n=4)	H+3	H+10	H+17	H+24	HF	hay	η_p²
1st quartile frequency (Hz)	3408.90 ± 238.59	3972.00 ± 174.84	3938.09 ± 158.34	3959.94 ± 174.67	3,9	4.599^*	0.61
5% frequency (Hz)	2678.48 ± 211.49	3151.92 ± 134.95	3104.71 ± 116.64	3110.98 ± 117.10	3,9	3.041^(*)	0.50
95% frequency (Hz)	5144.30 ± 379.10	5657.25 ± 269.51	5704.06 ± 282.06	5804.64 ± 299.91^a	3,9	4.970^*	0.62
Center frequency (Hz)	3885.87 ± 288.02	4471.15 ± 160.92	4443.06 ± 163.51	4504.88 ± 199.00	3,9	4.365^*	0.59
Maximum frequency (Hz)	4076.81 ± 247.94	4600.31 ± 150.85	4517.86 ± 102.38	4630.25 ± 124.46	3,9	3.375⁽*⁾	0.53
IQR bandwidth (Hz)	957.58 ± 108.04	990.28 ± 109.09	995.41 ± 70.72	1027.85 ± 98.30	3,9	0.192	0.06
90% bandwidth (Hz)	2465.81 ± 261.59	2505.31 ± 263.77	2599.33 ± 185.48	2693.64 ± 226.66	3,9	0.584	0.16

Average power (dB)	39.04 ± 2.20	50.08 ± 0.75^aa	50.10 ± 0.96^aa	49.72 ± 1.13^aa	3,9	14.690^***	0.83
Maximum power (dB)	71.80 ± 2.15	81.93 ± 0.70^aa	82.06 ± 0.96^aa	81.39 ± 1.15^aa	3,9	13.700^**	0.82
RMS amplitude (U)	76.91 ± 15.65	236.71 ± 20.59^aaa	242.19 ± 24.27^aaa	236.98 ± 29.44^aaa	3,9	21.42^***	0.88
Maximum amplitude (U)	681.08 ± 117.49	1877.33 ± 162.70^aaa	1973.63 ± 214.13^aaa	1920.89 ± 256.68^aaa	3,9	20.62^***	0.87

The mean ± SEM of each measure is presented for each time point. Last columns indicate the statistical results (F) of the one-way repeated measures ANOVA analyzing changes in time for each song parameter, the corresponding degrees of freedom (DF) and effect sizes.

^(*)

p<0.10

p<0.05

^**

p<0.01

^***

p<0.001

Significant differences compared to day 3 revealed by the Tukey post hoc tests are indicated in bold with ^a p<0.05, ^aa p<0.01, ^aaa p<0.001.