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. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: Nat Neurosci. 2020 Feb 17;23(3):327–336. doi: 10.1038/s41593-020-0589-7

Extended Data Fig. 1. Effect of αSyn pathology on GI health and cytokine expression.

Extended Data Fig. 1

a-d, GI function in WT, ASO, and aged mice is characterized by fecal pellet production (a; WT vs. ASO **p = 0.022, ASO vs. Aged **p = 0.0075), fecal pellet weight (b; WT vs. ASO ****p < 0.0001, WT vs. Aged ***p = 0.0009, ASO vs. Aged ****p < 0.0001), proportion fecal water weight (c; WT vs. ASO ****p < 0.0001, ASO vs. Aged ****p < 0.0001), and whole gut fecal transit time (d; WT vs. ASO ****p < 0.0001, ASO vs. Aged **p = 0.0038). e, Animal weight (WT vs. Aged ****p < 0.0001, ASO vs. Aged ****p < 0.0001). f, qPCR analysis of GBA1 expression in duodenum. Dots represent average of technical triplicates from a single animal. g, Densitometry analysis of cytokine panel from duodenal lysates after inoculation with α-Syn PFF or α-Syn monomer 7 dpi (Fractalkine **p = 0.0030, IL-1a **p = 0.0070, IL-6 *p = 0.0415, IL-7 * p = 0.0400, MCP-1 *p = 0.0416, MCSF *p = 0.0467, MIG *p = 0.0412, TECK *p = 0.0365, TIMP-2 *p = 0.0400). h, Representative cytokine panels detected by chemiluminescence. i, Representative Western blot immunostained for IL-6 and densitometry analysis normalized to WT (WT vs. PFF 60 dpi *p = 0.0327, 60 dpi PFF vs. monomer *p = 0.0480, WT vs. ASO *p = 0.0304). Data depicted are mean ± s.e.m. P values were determined by one-way ANOVA (a-f,i). The following n values represents number of independent animals used for statistical evaluation: e1a-c, WT = 42, ASO = 20, Aged = 19; e1d, WT = 13, ASO = 9, Aged = 12; e1e, WT = 42, ASO = 20, Aged = 19; e1f, WT = 3, ASO = 4, Aged = 4; e1g, monomer and PFF = 4 each; e1i, all conditions = 4 each.