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. Author manuscript; available in PMC: 2020 Jul 20.
Published in final edited form as: Nat Neurosci. 2020 Jan 20;23(3):423–432. doi: 10.1038/s41593-019-0573-2

Extended Data Fig. 2: Generation of an Htr2c-2A-iCre mouse line.

Extended Data Fig. 2:

(a) Htr2c-2A-iCre knock-in mice in which the coding sequences of Htr2c and iCre are linked by a viral peptide bridge 2A sequence. (b) Detection of wildtype and targeted Htr2c alleles by PCR genotyping. Similar results were obtained with at least 3 independent replications. (c) Immunohistochemical analysis of Htr2c-2A-iCre activity using a tdTomato reporter. Note high levels of tdTomato expression in the choroid plexus (CP). CC = corpus callosum. Similar results were obtained with at least 3 mice. Scale bar = 100 μm.