Extended Data Fig. 3: EYFP-expressing LPBN^Htr2c neurons are inhibited during sodium depletion.

(a) Cell-attached recordings taken from Htr2c-2A-iCre mice injected with AAV5-E1fa-DIO-EYFP. (b) Representative cell-attached recording of LPBN^Htr2c neurons after 7 days of control diet, or 7 days of sodium-deficient diet. (c) Firing frequency of LPBN^Htr2c neurons between control diet-fed mice (left) and sodium-deficient diet-fed mice (right). Two-tailed Mann-Whitney test. Control diet n = 21 cells, sodium-deficient diet n = 24 cells. Control diet 1.2 ± 0.3 Hz, Sodium-deficient diet 0.4 ± 0.2 Hz, P = 0.007. All data represented as mean ± s.e.m. **P < 0.01.