Figure 3.

Knockdown of lncRNA PVT1 minimized glycolysis of PDAC cells. PDAC cells were cultured for 24 h after 48 h of transfection with either control or si-PVT1#3. Glucose uptake (A), lactate secretion (B) and intracellular ATP levels (C) of these cells were quantified and normalized for cell numbers. Shown data are mean ± SD (n = 3). Cell lysates were then analyzed by western blotting by anti-HIF-1A, anti-LDHA, anti-GULT1, anti-HK2 or anti-β-actin antibodies (D). (^**P < 0.01, ^***P < 0.001)