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. 2020 Feb 14;11(9):2572–2579. doi: 10.7150/jca.37959

Figure 5.

Figure 5

PVT1 inhibits miR-159-3p and then positively regulates HIF-1A. (A) Bioinformatics analysis showed the prediction for miR-159-3p binding sites on HIF-1A. (B) The luciferase reporter constructs containing the wildtype (HIF-1A3'UTR-WT) or mutant HIF-1A (HIF-1A3'UTR-MUT) sequence. HIF-1A3'UTR-WT or HIF-1A3'UTR-MUT was co-transfected with miR-159-3p mimics or NC mimics for 48 hours. (C and D) The mRNA and protein levels of HIF-1A transfected with miR-519d-3p-mimic, pc-PVT1 and their controls were measured by qRT-PCR and western blot assays. (E) Correlation analysis between HIF-1A and miR-159-3p were detected in the cancer tissue of PDAC patients. (** and ## P < 0.01, ***, ### and @@@P < 0.001))