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. 2020 Feb 10;11(9):2408–2420. doi: 10.7150/jca.35372

Figure 3.

Figure 3

Effect of CDCA5 on the proliferation and regulation of BC cells. (A) Knockdown of CDCA5 inhibited the proliferation of 5637 and T24 cell lines, while overexpression of CDCA5 promoted the proliferation of the UMUC3 cell line as determined by a cell counting kit-8 assay. (B) Colony formation assays were used to detect the proliferation ability of CDCA5 knockdown or overexpression BC cell lines. (C) Flow cytometry was used to calculate the cell cycle distribution following treatment with sh-NC, sh-CDCA5-1, and sh-CDCA5-2 in the 5637 and T24 cell lines and OE-NC and OE-CDCA5 in the UMUC3 cell line. *P<0.05 vs. sh-NC or OE-NC, **P<0.01 vs. sh-NC or OE-NC, ***P<0.005 vs. sh-NC or OE-NC. ns, not significant. (D) Effect of CDCA5 knockdown and overexpression on the expression of cell cycle-related proteins by Western blotting.