Figure 4.
Viremia following CHIKV-NoLS CAF01 inoculation and CAF01 induced footpad swelling in C57BL/6 mice. C57BL/6 mice were inoculated with viable virus particles (104 pfu CHIKV-WT or CHIKV-NoLS), CAF01 delivered RNA (2 μg CHIKV-WT or CHIKV-NoLS RNA complexed with 0.3 mg CAF01) or RNA alone (2 μg CHIKV-WT or CHIKV-NoLS RNA) subcutaneously in the ventral/lateral side of the right foot. Mock inoculated mice received 20 μl VP-SFM media alone or VP-SFM media containing 0.3 mg CAF01. (A) Serum was collected on days 1–5 post inoculation and virus titrated by plaque assay. Mice were monitored daily for signs of CHIKV-induced footpad swelling in the (B) ipsilateral (inoculated) and (C) contralateral (non-inoculated) foot. Each symbol represents the mean ± standard error from five to six mice.