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. 2020 Mar 5;10:296. doi: 10.3389/fonc.2020.00296

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Copyright © 2020 Garcia, Uluisik, van Linden, Jones, Venkataraman, Vibhakar and Porter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Altered H3K4 trimethylation contributes to AZD1775 resistance. (A–C) AZD1775-naive and -resistant Molm13 (A), Jurkat (B), and REH (C) cells were treated with panobinostat (10 nM), vorinostat (1 μM), and/or AZD1775 (0.5 or 1 μM) for 24 h after which protein lysates were subjected to western blotting with antibodies specific to H3K4me3, histone H3, KDM5A, and actin. (D–F) AZD1775-naive and -resistant Molm13 (D), Jurkat (E), and REH (F) cells were treated with the indicated concentrations of CPI-455 and/or 50–2,000 nM AZD1775 for 72 h. Viable cell counts are normalized to cells receiving no AZD1775 treatment (NT). Results are displayed as mean ± SEM from three independent experiments. *P < 0.05, ****P > 0.0001.