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IFNγ− T cell clones have 5′methylcytosine-guanine (CpG) methylation in the interferon gamma (IFNγ) promoter. Proportion of DNA sequences that have CpG methylation at positions −51 and −186 (A) as well as at positions −295, +122, and +128 (B). (C) The number of methylated sites within the IFNγ promoter correlates with the proportion of IFNγ− cells for each T cell clone. N, number of experimental repeats = 3. Analysis performed with non-parametric Spearman correlation test.
