TABLE 1.
List of mt-dark proteins identified in HeLa dataset.
| Accession | Description | Mito evidence IMPI | Mito localization IMPI | HPA cell localization | Enzyme | Exp. fraction | Gravy score |
| NX_O60941-1 | Dystrobrevin beta | Predicted | Unknown | Mitochondria (A) | Try; Glu-C | F1, F2 | −0.55 |
| NX_Q3SXM5-1 | Inactive hydroxysteroid dehydrogenase-like protein 1 | Known | OM | Intracellular, Membrane (P) | Chym; Try | F2 | +0.15 |
| NX_Q4VC31-1 | Coiled-coil domain-containing protein 58 | Known | IMS | Nucleoli, Mitochondria (A) | Try; Chym; Glu-C | F2 | −0.60 |
| NX_Q56VL3-1 | OCIA domain-containing protein 2 | Known | Unknown | Mitochondria (E) | Try | F2 | −0.26 |
| NX_Q8IYQ7-1 | Threonine synthase-like 1 | Known | Matrix | Nuclear bodies (A), Mitochondria (A), Cytosol (A) | Try | F2 | −0.13 |
| NX_Q8NFV4-1 | Protein ABHD11 | Known | Matrix | Mitochondria (S) | Try; Chym | F2 | −0.09 |
| NX_Q96EX1-1 | Small integral membrane protein 12 | Predicted | Unknown | Mitochondria (A) | Try | F2 | −0.53 |
| NX_Q96C01-1 | Protein FAM136A | Known | IMS | Mitochondria (A) | Try; Glu-C | F2 | −0.43 |
| NX_Q96ER9-1 | Coiled-coil domain-containing protein 51 | Known | Matrix | Nucleosome (S), Mitochondria (S) Centrosome (A) | Try; Glu-C | F2 | −0.38 |
| NX_P56378-1 | 6.8 kDa mitochondrial proteolipid | Known | IM | Mitochondria (S),Nucleoli (S) | Try | F1,F2 | −0.02 |
| NX_Q9GZT6-1 | Coiled-coil domain-containing protein 90B | Known | Matrix | Mitochondria (E) | Try; Glu-C | F2 | −0.55 |
| NX_A8MTT3-1 | Protein CEBPZOS | Known | IMS | Nucleoplasm (A) | Try; Glu-C | F2 | −0.27 |
| NX_Q9H4I3-1 | TraB domain-containing protein | Known | OM | Nucleus (A), Mitochondria (A) | Try | F2 | −0.21 |
| NX_Q9UFN0-1 | Protein NipSnap homolog 3A | Known | Matrix | not available | Try; Glu-C | F2 | −0.37 |
| NX_Q6P1 × 6-1 | UPF0598 protein C8orf82 | Known | Matrix | Nucleus (A) | Try; Chym | F2 | −0.23 |
| NX_Q8N2U0-1 | Transmembrane protein 256 | Predicted | Unknown | Vesicles (A) | Try | F2 | +0.46 |
| NX_Q8WVI0-1 | Small integral membrane protein 4 | Predicted | Unknown | Nucleoplasm (A), Mitochondria (A) | Try | F2 | −0.54 |
| NX_Q8WW59-1 | SPRY domain-containing protein 4 | Known | Matrix | Nucleoplasm (A) | Try | F1,F2 | −0.07 |
| NX_Q96BQ5-1 | Coiled-coil domain-containing protein 127 | Known | OM/IMS | Nucleus (S), Nucleoli (S) | Try; Chym | F1,F2 | −0.72 |
| NX_Q96DB5-1 | Regulator of microtubule dynamics protein 1 | Known | OM/IMS | Centrosomes (S),Actin filaments (S) | Try | F1,F2 | −0.37 |
| NX_Q96KF7-1 | Small integral membrane protein 8 | Known | OM | Vesicles (A) | Try; Chym | F2 | −0.55 |
| NX_Q9NU23-1 | LYR motif-containing protein 2 | Known | matrix | Cytosol (A) | Try | F2 | −0.70 |
Accession, neXtprot accession number of uPE1 mt-dark protein; Description, extended protein name; MitoEvidenceIMPI. Mitochondrial protein classification; MitoLocalization IMPI, protein localization within mitochondria according to IMPI; HPA cell localization, main protein location Approved, Predicted, Enhanced or Supported in Human Protein Atlas project; Enzyme, protease used to generate the identified proteolytic peptides; Experimental Fraction, experimental sample in which the corresponding protein has been retrieved; Gravy Score, Grand Average of Hydropathy, measure the hydrophobicity of a specific protein.