Table 2.
Plasmids used in the study.
| Plasmid | Genotype | Reference |
|---|---|---|
| pACBSCE | Para controlled λ-Red recombinase | 39 |
| pDOC-K | bla flanked by FLP | 39 |
| pCP20 | bla cat cI857 repA(ts) PR::flp | 72 |
| pREN126 | pDOC-K-336bp upstream pal (ΔAgeI)-pal-GGGGS-mCherry (ΔAgeI)(ΔEcoRI)-bla-300 bp-downstream pal | This study |
| pNP4 | pBR322-GFP-t-tolA | 21 |
| pREN88 | pBR322-GFP-t-tolA(H22A) | 37 |
| pDAB17 | pBAD24-tolB | 18 |
| pGP3 | pBAD24-tol-HIS6 | This study |
| pGP89 | pBAD24ptol(H146A)-HIS6 | This study |
| pGP90 | pBAD24-tol(D150A)-HIS6 | This study |
| pGP95 | pBAD24-tol(T165A)-HIS6 | This study |
| pREN71 | pBAD24-tol(L25A) | This study |
| pREN72 | pBAD24-tol(L25A, I27A) | This study |
| pREN70 | pBAD24-tol(L25I, I27A) | This study |
| pREN73 | pBAD24-tol(L25A, V26A, I27A) | This study |
| pREN28 | pQE-Im9-FXa- P. aeruginosa TolA | This study |