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. 2020 Mar 11;10:4472. doi: 10.1038/s41598-020-61433-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CDR 3 loop ensemble of the hierarchical clustering of the 24.1 µs 1BEC trajectories, the 9.5 µs 2APB trajectories, the 7.0 µs 2APT trajectories, the 8.8 µs 2APF trajectories and the 4.8 µs 2APX trajectories. Even though the CDR 3 loop is not directly involved in binding it is still rigidified during affinity maturation.