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. 2020 Mar 11;11:1315. doi: 10.1038/s41467-020-14958-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Sema7a^−/− animals and littermate controls were evaluated by magnetic resonance tomography (MRT) to exclude anatomical and functional alterations in Sema7a^−/− animals (n = 6/group). b Left and right ventricular ejection fraction (LVEF or RVEF in %) determined through MRT (n = 6/group). c Sema7a^−/− mice and littermate controls were exposed to 60 min of ischemia and 120 min of reperfusion with samples taken after 1 or 120 min of reperfusion. Representative TTC-stained slices of myocardial tissue showing infarcted area (blue/dark = retrograde Evans blue staining; red and white = AAR, white = infarcted tissue, 120 min) with d systematic evaluation of infarct sizes (n = 7;6) and corresponding troponin I plasma levels (n = 6;8). e Representative histological sections (scale bar 100 µm) of Sema7a^−/− and littermate control hearts (120 min). f Number of PNCs in myocardial tissue sections in AAR of Sema7a^−/− mice and littermate controls (120 min, n = 9/group). g Representative flow-cytometric plots of PNCs in the blood of Sema7a^−/− and littermate controls, showing GPIb (CD42b) and P-selectin (CD62P) after 1 and 120 min reperfusion. Mean fluorescence intensity (MFI) for h GPIb (CD42b, n = 6;7;7;7;7) and i P-selectin (CD62P, n = 7/group) in PNCs and j systematic evaluation of PNCs in % by flow cytometry in the blood of Sema7a^−/− animals and littermate controls (n = 7/group). k Representative flow-cytometric plots of PNCs in the AAR and MFI for l GPIb (CD42b, n = 7;7;7;7;6) and m P-selectin (CD62P, n = 7;6;7;7;7) and n systematic evaluation of PNCs by flow cytometry in % in the AAR of WTs and Sema7a^−/− animals at 1 min and 120 min (n = 6;7;7;7;7). Comparisons in b, d, f were analyzed by unpaired two-tailed Student’s t-tests (data are mean ± SD). For h–j, l–n we used log transformation of data to conform normality. For log-transformed data, unpaired two-tailed Student’s t-tests were performed on the log values and results are displayed as geometric means and their 95% confidence intervals. *p < 0.05, **p < 0.01, and ***p < 0.001 as indicated.