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. 2020 Mar 11;11:1315. doi: 10.1038/s41467-020-14958-x

Fig. 6. Anti-Sema7a reduces MIRI, platelet activation and PNC formation.

Fig. 6

Animals were injected with either anti-semaphorin 7A antibody (anti-Sema7a) or IgG control 5 min before starting 120 min reperfusion after 60 min of ischemia, with samples taken after 1 or 120 min of reperfusion. a Representative TTC-stained heart slices of myocardial infarcts (blue/dark = retrograde Evans blue staining; red and white = AAR, white = infarcted tissue) with b systematic evaluation of infarct sizes (n = 6/group) and correlating troponin I plasma levels (n = 6;8). c Representative histology sections (scale bar 100 µm) of WT animals injected with either IgG control or anti-Sema7a and d number of PNCs counted from myocardial tissue sections (n = 9/group). e Representative flow cytometry plots of PNCs in the blood of Sham, anti-Sema7a-injected or IgG control-injected animals, expressing GPIb (CD42b) and P-selectin (CD62P). Systematic evaluation of flow-cytometric expression of mean fluorescence intensity (MFI) for f GPIb (CD42b, n = 5;4;3;4;5) and g P-selectin (CD62P, n = 6;4;4;3;5) and h systematic evaluation of PNCs in % by flow cytometry in the blood of animals injected with anti-Sema7a or IgG control at 1 and 120 min (n = 3;4;4;3;4). i Systematic evaluation of flow-cytometric expression of MFI for j GPIb (CD42b n = 4;5;3;3;4) k P-selectin (CD62P, n = 4/group) and l systematic evaluation of PNCs in % by flow cytometry in the AAR of animals injected with anti-Sema7a or IgG control at 1 and 120 min (n = 5;6;5;4;4). Comparisons in b, d were analyzed by unpaired two-tailed Student’s t-tests (data are mean ± SD). For f–l we used log transformation of data to conform normality. For log-transformed data, unpaired two-tailed Student’s t-tests were performed on the log values and results are displayed as geometric means and their 95% confidence intervals. *p < 0.05, **p < 0.01, and ***p < 0.001 as indicated.