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. 2020 Mar 11;11:1312. doi: 10.1038/s41467-020-14999-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Lactate concentrations in 5 dpf WT and mzKO larvae. Data represent mean and SEM of four independent clutches each with 50 larvae per genotype. p-values from two-sided paired t-test. b Citric acid cycle organic acid concentrations in 5 dpf WT and mzKO larvae. Data represent mean and SEM of four independent clutches each with 50 larvae per genotype. p-values from two-sided paired t-test with Bonferroni correction. *α-KG concentrations are a 10-fold multiple to enable visualization on the same scale. c Distributions of the export rate of lactate and succinate (5000 flux samples) comparing the unconstrained MitoCore model versus the CIII-blocked model derived by MitoCore flux balance analysis (FBA). d Venn diagram depicting intersections of proteins identified in 4 co-IP/mass spec experiments of BR in HEK293T mitochondria. Endogenous BR and overexpressed untagged BR were immunoprecipitated with α-BR, overexpressed BR-FLAG with α-FLAG. e Immunoprecipitation of BR-FLAG followed by immunoblotting for indicated ETC proteins from mouse mitochondria with AAV9-mediated expression of BR-FLAG. MTCYB, UQCRFS1, UQCRC1, and UQCRH are components of CIII. NDUFA9 and COX4 are components of CI and CIV, respectively. f. Reciprocal IP of BR with CIII components. UQCRQ-FLAG and BR-HA were co-transfected into HEK293T. UQCRQ-FLAG immunoprecipitation was followed by immunoblotting for BR-HA and indicated proteins. CS is an unrelated matrix protein.