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. 2020 Mar 11;11:1304. doi: 10.1038/s41467-020-15067-5

Fig. 4. The combination between lopinavir and nelfinavir with PERK inhibitors elicits sERr, reduces total P-Tyr levels and compromises viability of RTK-addicted cells similar to TKIs.

Fig. 4

a Immunoblotting against c-MET, EGFR, and p97 and ponceau-s staining of HepG2 cells treated with Tg, nelfinavir (20 μM), and ISRIB alone or in combinations for 18 h. b Immunoblotting against c-MET, EGFR, and p97 for HepG2 treated with DMSO or lopinavir (20 μM) with different concentrations of ISRIB for 18 h; c HCC827 WT cells where treated with osimertinib (1 μM), GSK515 (0.5 μM), lopinavir (26 μM), and nelfinavir (16 μM) alone in combination for 32 h. Cells where then analyzed by immunoblotting for p-EGFR, EGPR, p-MET, c-MET, p-Tyr, p97. Shown is one of two independent repetitions. d Cells were analyzed by flow cytometry to determine viability (shown in red). Bars represent the percentage of live cells. e HMC-1.1 cells where treated with dasatinib (0.5 μM), GSK515 (0.5 μM), lopinavir (26 μM), and nelfinavir (16 μM) alone in combination for 32 h. Cells where then analyzed by immunoblotting for p-KIT, KIT, p-Tyr, p97. Shown is one of two repetitions. f Cells were analyzed by flow cytometry to determine viability (shown in red). Bars represent the percentage of live cells.