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. 2020 Mar 11;11:1306. doi: 10.1038/s41467-020-15090-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Precursors and their fragment ions used for quantification of Ub and Ub^KEKS. b Fragment ion chromatogram of Ub and Ub^KEKS in WT and Ub^KEKS KO (clone 4.3) HeLa cells in lamin A (LMNA) and lamin B2 (LMNB2) pulldown assays. Blue line represents heavy (AQUA) peptide, red line represents endogenous peptides with m/z values indicated in the box above chromatograms. Dotted lines indicate peak boundaries with black arrows showing peaks. Peak values with mass error are indicated above each peak. c Ratio of Ub^KEKS to Ub in WT and Ub^KEKS KO (clone 4.3) HeLa cells in LMNA and LMNB2 pulldown assays. Data are presented as mean values ± SD; individual data points are superimposed on the bar graph. Statistical analysis was performed using unpaired two-tailed t-test (n = 4 independent experiments). Source data are provided as a Source Data file.