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. 2020 Mar 11;6:12. doi: 10.1038/s41420-020-0246-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Fluorescent staining and flow cytometry analysis of cellular lysosomal levels in 10 different human PDAC cell lines (five classical and 5 mesenchymal) in three independent experiments. b Protein expression analysis for key lysosomal marker (LAMP1, LAMP2, and CTSD) in representative human PDAC cell lines with and without exposure to trametinib. Blot is representative of two independent experiments. c Transcript expression quantification (qPCR) of the lysosomal marker LAMP1 (upper panel and the lysosomal hydrolase CTSD (lower panel) in two representative human PDAC cell lines. triplicates of two independent experiments are shown. d, e representative protein expression blot and transcript expression (qPCR) of lysosmal ATPases (ATPV0D1 & ATP6V1A), respectively with and without trametinib exposure in two representative human PDAC cell lines. f Immunofluorescence staining of the lysosomal master regulator TFEB in two human PDAC cell lines with and without trametinib treatment. Images are representative of 2 independent stainings.