Skip to main content
. 2020 Mar 5;11:176. doi: 10.3389/fimmu.2020.00176

Figure 3.

Figure 3

Neoantigen screening through an inventory-shared neoantigen library. The TCGA and COSMIC databases are used to mine high-frequency mutant genes in nine types of human malignant solid tumors to construct the neoantigen library. Data from the patient who underwent targeted sequencing were compared with those in the neoantigen library to identify the specific neoantigen in the patient's tumor cells. The selected neoantigen will be pulsed and presented by APCs in the context of the patient's MHC. The coculture of the patient's T cells with the APCs can be used to identify the neoantigen presented by the APCs. The detection of immunogenic neoantigens for tumor recognition is performed by ELISPOT-based cytokine detection secreted from activated T cells when they recognize the target neoantigen.