Table 2.

Advantages and disadvantages of different approaches for neoantigen identification.

Approach	Advantage	Disadvantage
Whole exome sequencing combined with mass spectrometry	High throughput Identify post-translational modification	Many false positives
Neoantigens screening through inventory-shared neoantigens library	Labor and time saving	Depend on the capacity of shared antigen database
Neoantigen screen via trogocytosis	Identify the neoantigen and TCR simultaneously	Hard to manipulate, especially construct SCT cDNA database and transduce into cancer cells