Figure 4.

Elevation of lncRNA NEAT1 Downregulates miR-339-5p to Increase the Expression of HOXA1 and Neuronal Cell Apoptosis during Hypoxia-Ischemia

(A) The binding site between lncRNA NEAT1 and miR-339-5p predicted by starBase database. (B) The mRNA expression of HOXA1 in neuronal cells measured by qRT-PCR. (C) The binding between miR-339-5p and lncRNA NEAT1 by dual-luciferase reporter gene assay. (D) The expression of miR-339-5p and HOXA1 in neuronal cells measured by qRT-PCR. (E) The protein expression of HOXA1 in neuronal cells normalized to β-actin determined by western blot analysis. (F) The expression of lncRNA NEAT1 in OGD-treated neuronal cells in response to lncRNA NEAT1 knockdown. (G) The apoptosis of OGD-treated neuronal cells detected by TUNEL staining (original magnification ×200). (H) The expression of neuronal cell apoptosis-related proteins normalized to β-actin as determined by western blot analysis. *p < 0.05 versus untreated neuronal cells or neuronal cells treated with sh-NEAT1. The measurement data were expressed as mean ± standard deviation. Data between two groups were analyzed using unpaired t test, and data among multiple groups were tested using one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated three times independently.