Figure 1.
Autophagy Maintains AT2 Cell Pool during BLM-Induced Lung Injury
(A) Atg5 gene expression in mouse AT2 cells after BLM injury (n = 6).
(B) Survival of Atg5f/f, SftpcCreER;Atg5f/f (pretreated with tamoxifen), or Nkx2.1Cre;Atg5f/f mice after intratracheal instillation of BLM (n = 10).
(C) Hematoxylin/eosin staining (left column) and Masson trichrome (right column) staining of lung sections from Atg5f/f, SftpcCreER;Atg5f/f (pretreated with tamoxifen), and Nkx2.1Cre;Atg5f/f mice after BLM injury. Scale bar: 50 μm.
(D and E) Representative charts of flow cytometric analysis (D) and summarized abundance (E) of survived AT2 cells in lungs of Atg5f/f, SftpcCreER;Atg5f/f (pretreated with tamoxifen), or Nkx2.1Cre;Atg5f/f mice after BLM injury (n = 4). Data are representative of two or more independent experiments with error bars representing the mean ± SD. ∗p < 0.05.