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. 2020 Feb 13;14(3):420–432. doi: 10.1016/j.stemcr.2020.01.008

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Autophagy Maintains AT2 Cell Pool during BLM-Induced Lung Injury

(A) Atg5 gene expression in mouse AT2 cells after BLM injury (n = 6).

(B) Survival of Atg5^f/f, Sftpc^CreER;Atg5^f/f (pretreated with tamoxifen), or Nkx2.1^Cre;Atg5^f/f mice after intratracheal instillation of BLM (n = 10).

(C) Hematoxylin/eosin staining (left column) and Masson trichrome (right column) staining of lung sections from Atg5^f/f, Sftpc^CreER;Atg^5f/f (pretreated with tamoxifen), and Nkx2.1^Cre;Atg5^f/f mice after BLM injury. Scale bar: 50 μm.

(D and E) Representative charts of flow cytometric analysis (D) and summarized abundance (E) of survived AT2 cells in lungs of Atg5^f/f, Sftpc^CreER;Atg5^f/f (pretreated with tamoxifen), or Nkx2.1^Cre;Atg5^f/f mice after BLM injury (n = 4). Data are representative of two or more independent experiments with error bars representing the mean ± SD. ^∗p < 0.05.