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. 2020 Feb 13;14(3):420–432. doi: 10.1016/j.stemcr.2020.01.008

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Autophagy Sustains the Proliferative Capacity of AT2 Cells during BLM Injury

(A) CFEs of mouse AT2 cells isolated from untreated or BLM-injured Atg5^f/f mice at day 10 after seeding (n = 4).

(B) Representative micrographs of organoid cultures of mouse AT2 cells isolated from Atg5^f/f, Sftpc^CreER;Atg5^f/f (pretreated with tamoxifen), or Nkx2.1^Cre;Atg5^f/f mice 14 days after BLM injury. Scale bar: 200 μm.

(C and D) CFEs (C) and sizes (D) of organoid colonies from Atg5^f/f, Sftpc^CreER;Atg5^f/f (pretreated with tamoxifen), or Nkx2.1^Cre;Atg5^f/f mice at day 14 after BLM injury (n = 4).

(E and F) (E) Immunostaining of organoid colonies and (F) quantification of fractions of Ki67⁺pro-SPC⁺ cells in total pro-SPC⁺ cells in AT2 organoids (n = 4). Data are representative of three independent experiments with error bars representing the mean ± SD. ^∗p < 0.05.