Figure 4.

Genetic Deletion of Glut1 Inhibits AT2 Cell Proliferation

(A) CFEs of AT2 cells isolated from Atg5^f/f or Sftpc^CreER;Glut1^f/f mice at steady state (n = 5).

(B) Colony sizes of organoid cultures of AT2 cells isolated from Atg5^f/f or Sftpc^CreER;Glut1^f/f mice (n = 5).

(C) Sftpc mRNA expression in organoid cultures (n = 5).

(D) T1α and Aqp5 mRNA expression in organoid cultures (n = 5).

(E and F) Representative charts of flow cytometric analysis (E) and summarized abundance (F) of AT2 cells in Sftpc^CreER;Glut1^f/f mice after BLM treatment relative to littermate controls (n = 5).

(G) CFEs of AT2 cells isolated from Atg5^f/f or Sftpc^CreER;Glut1^f/f mice after BLM treatment (n = 5).

(H) Immunostaining of organoid cultures and quantification of Ki67⁺pro-SPC⁺ cells as a proportion of total pro-SPC⁺ cells in organoid cultures from Atg5^f/f or Sftpc^CreER;Glut1^f/f mice after BLM treatment (n = 5). Data are representative of three independent experiments with error bars representing the mean ± SD. ^∗p < 0.05, ^∗∗p < 0.01.