Figure 6.

Fatty Acid Synthesis Pathway Negatively Influences AT2 Cell Proliferation

(A) Schematic of intermediates and their inhibitors in fatty acid synthesis pathways.

(B) CFE of mouse AT2 cultures with and without BMS (n = 5).

(C) Immunostaining of organoid cultures and quantification of Ki67⁺pro-SPC⁺ cells in total pro-SPC⁺ cells in organoid cultures in the presence and absence of BMS (n = 5).

(D) CFE of AT2 cell cultures with and without orlistat (n = 5).

(E) Immunostaining of organoid cultures and proportions of Ki67⁺pro-SPC⁺ cells in total pro-SPC⁺ cells in organoid cultures in the presence and absence of orlistat (n = 5).

(F and G) After intratracheal instillation of BLM, Nkx2.1^Cre;Atg5^f/f mice were intraperitoneally injected with PBS or orlistat. At 14 days after BLM, lungs were harvested for hematoxylin/eosin (F) and Masson trichrome (G) staining. Scale bar: 50 μm. Data are representative of two independent experiments with error bars representing the mean ± SD. ^∗p < 0.05, ^∗∗p < 0.01.