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. 2020 Mar 11;10:4509. doi: 10.1038/s41598-020-60233-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rapid extravasation into the skin but delayed localization at the dermal-epidermal junction, of LysM-eGFP⁺ cells following anti-COL7 IgG injection. (a) Graph indicates the number of all LysM-eGFP⁺ cells identified in the dermal compartment. The bars correspond to the LysM-eGFP⁺ cells in the unscratched and scratched ears on day 4 after the initial normal rabbit IgG (NR IgG) i.v. injection and the lines correspond to the number of LysM-eGFP⁺ cells in the mice injected with anti-COL7 IgG on day 0, 1 and 4. The data are based on 6–8 evaluated stacks from at least 3 independently performed experiments [*p < 0.05, ANOVA on Ranks, followed by Dunn’s Method for comparison to reference (unscratched, normal rabbit IgG, day 4)]. (b) Here, only cells located at the dermal-epidermal junction were counted. (c) Total number of LysM-eGFP⁺ cells in the dermis and (d) number of LysM-eGFP⁺ cells located at the dermal-epidermal junction of mice i.v. injected with anti-COL7 IgG increases over the 8 day observation period. The data are based on experiments in 6 mice, and are presented as means and standard error for presentation purposes. (*p < 0.05, ANOVA on Ranks with Dunns’ Method, compared to day 1). (e) Representative stacks of the ears, generated from the images obtained by multiphoton microscopy.