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. 2020 Mar 11;10:4481. doi: 10.1038/s41598-020-61432-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Processivity on duplex RNA substrates with different duplex lengths. (A,B): The single-turnover unwinding of duplex RNA substrates with different lengths of duplex. (A) Gel retardation assay of nsP13 and duplex RNAs with different duplex lengths. The substrates of 15 bp (15D), 20 bp (20D), and 25 bp (25D) duplexes containing a 5′-poly(U) tail of 20 bases (20U) were designed as shown in Supplementary Table 1. The unwinding products were resolved by non-denaturing 15% PAGE. (B, C) The amplitudes were as follows: 15 bp (●: 20U/15D RNA) = 0.98 ± 0.03, 20 bp (▽: 20U/20D RNA) = 0.89 ± 0.02, and 25 bp (◆: 20U/25D RNA) = 0.31 ± 0.02.