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. 2020 Mar 11;10:4481. doi: 10.1038/s41598-020-61432-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Unwinding of duplex RNA substrates with 5′-ss tails of different lengths. (A,B): Single-turnover unwinding of 25 bp (25D) RNA substrates with 5′-poly(U) tails of different lengths by helicase nsP13. (A) Gel retardation assay of nsP13 and duplex RNA substrates with 5′-ss tails of different lengths. Three different substrates containing 25 duplex-fixed (25D) and the 5′-poly(U) tail of 20 bases (20U), 25 bases (25U), and 30 bases (30U) were designed as shown in Supplementary Table 1. The unwinding products were resolved by non-denaturing 15% PAGE. (B,C) The amplitudes were as follows: 20U (●: 20U/25D RNA) = 0.63 ± 0.01, 25U (▽: 25U/25D RNA) = 0.6 ± 0.01, and 30U (◆: 30U/25D RNA) = 0.19 ± 0.01.