Figure 2.

Resident macrophage-like cells in mouse cochlea. (A) P4 Cx3cr1^GFP/+ cochleae have GFP⁺ cells scattered throughout the cochlea, including the auditory nerve (AN), spiral ganglion (SG), basilar membrane (BM), stria vascularis (SV), and spiral ligament (SL). Phalloidin (red) and DAPI (blue) label F-actin and nuclei, respectively. (B) Lateral wall of a P4 Cx3cr1^GFP/+ cochlea with GFP⁺ cells mainly localized around blood vessels (blue, labeled with anti-CD34 antibody). SV, stria vascularis; SP, spiral prominence; OS, outer sulcus; scale bars, 100 μm. Reproduced from Figure 7, Nakanishi et al. (6). GFP⁺ cells are similarly distributed in all parts of the cochlea at P30 (6).