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. 2020 Mar 5;14:145. doi: 10.3389/fnins.2020.00145

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Copyright © 2020 Brozzetti, Sacchetto, Cecchini, Avesani, Perra, Bongianni, Portioli, Scupoli, Ghetti, Monaco, Buffelli and Zanusso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pattern of immunolabeling by olfactory marker protein (OMP) and β-tubulin III (TUJ-1) in cell obtained by olfactory brushing. Sample harvested by olfactory brushing and analyzed following centrifugation. Double immunostaining with OMP (green) and TUJ-1 (red). Several cells with different morphology showed a cytosolic positivity to OMP. Most of round cells and non-neural-shaped cells show an intense positivity in the cytoplasm, while olfactory neurons show a faint granular pattern. In contrast, TUJ-1 stains mainly the neural processes, in particular, the axonal hillock of cells, identified as olfactory neurons [outlined square in (A) details in (B)]. Weak cytosolic positivity is also observed in the apical portion of supporting-like cells. Scale bar (A): 20 μm. Scale bar (B): 10 μm.