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. 2020 Mar 5;14:145. doi: 10.3389/fnins.2020.00145

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Copyright © 2020 Brozzetti, Sacchetto, Cecchini, Avesani, Perra, Bongianni, Portioli, Scupoli, Ghetti, Monaco, Buffelli and Zanusso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunocytochemical analysis of a cytocentrifuged sample of olfactory mucosa (OM) using OMP (green) and PCK (red). While OMP stains round and non-neuronal-shaped cells, PCK preferentially stains the whole apical dendritic projection of olfactory neurons [outlined square (A) up to the cilia boundary (detail B)]. Interestingly, in ONs, the immunopositivity with PCK is distributed on the opposite side of that obtained with β-tubulin III. In the other cells, PCK expression is distributed on the boundary of the cell body, all along the plasma membrane (arrows) of cells that have a round shape. Scale bar (A): 20 μm. Scale bar (B): 10 μm.