Figure 4.

MERTK Ligands Rather Than αVβ5 Integrin Ligands Stimulate POS Ensheathment by Human RPE

(A) A schematic of the experiment.

(B–D) SEM image of cells treated with F-POS and 30% serum (B), 5 μg/mL GAS6 (C), or 5 μg/mL MFGE8 (D). Scale bar, 1 μm.

(E–J) Ten images containing around ten cells from each condition were analyzed. Data are represented as means ± SD. N = 3 biological repeats. (E–J) Quantification of the number of sheets associated with POS per cell, the percentage of cells presenting sheets, and the number of unensheathed POS per cell in the different conditions. Significance was calculated using one-way ANOVA test. ns > 0.05, ^∗p < 0.05, ^∗∗ p< 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001. Data are represented as means ± SD. N = 3 biological repeats. Related to Figure S3.