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. 2019 Dec 27;200(1):45–52. doi: 10.1111/cei.13406

Figure 1.

Figure 1

Cisplatin‐induced programmed cell death ligand‐1 (PD‐L1) expression in A2780cis cells and contributed to T lymphocyte disfunction. (a) Difference of relative expression of PD‐L1 between A2780 and A2780cis by real‐time polymerase chain reaction (PCR) (up) and Western blot (down). (b) An optimal concentration of cisplatin‐induced PD‐L1 maximal expression in A2780cis cells by Western blot assay. (c,d) Conditioned media from the cisplatin‐treated A2780cis was used to culture CD8+  T lymphocytes. Media from H2O‐treated A2780cis was used as negative control. PD‐1 antibody or mouse immunoglobulin (Ig)G was used to block the PD‐1‐dependent pathway on T cells. Effects of the conditioned media on CD8+ T lymphocyte proliferation and apoptosis were evaluated by cell count kit‐8 (c) and flow cytometry (d), respectively. * P < 0·05 and ** P < 0·01 versus A2780cis; ▲ P < 0·05 versus A2780cis + cisplatin + IgG.