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Phylogeny-based taxonomy assignment. A: Building a reference tree for the phylogeny-based taxonomy assignment to 16S rRNA marker gene OTUs: from the latest edition of Silva SSU, all entries referring to Bacteria and Archaea were used and using the “art” algorithm, 10,000 consensus taxa were kept. B: Using PaPaRa and the OTUs that come up from every analysis, an MSA was made and EPA-ng took over the phylogeny-based taxonomy assignment. *noun project icons by: Rockicon and A Beale.
