Figure 6.

At-EE induced ER stress to suppress NF-kB/p-STAT3/IL-6 and VEGF loop. (a) Cells were treated with At-EE at 5 or 10 μg/ml for 48 h, and GRP78 and CHOP were analyzed by western blot. (b) Cells or cells transfected with specific CHOP siRNA were treated by At-EE or DMSO, and NF-kB and p-STAT3 were analyzed by western blot. (c) Cells or cells transfected with specific CHOP siRNA were treated by At-EE or DMSO, and IL-6 and VEGF in conditioned media were detected by ELISA kits. (d) The expressions of p-STAT3, NF-kB, CHOP, VEGF, and IL-6 were detected by immunohistochemical staining. (e) Schematic model illustrating the potential pathway associated with At-EE inhibiting ovarian cancer cells. The results were similar in at least three independent experiments. ^∗p < 0.05. ^∗∗p < 0.01.