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4-HBA-induced autophagy was required for the intracellular restriction of T. gondii in BMDMs. (A) Immunofluorescence microscopy on LC3 puncta formation in BMDMs treated with 4-HBA. Representative images (top; Scale bar=10 μm). LC3 punctate foci per cell (Lower panel). (B) SAG1 mRNA level guantifed by rt-PCR. BMDMs were infected with T. gondii RH strain, treated with 3-MA (10 μM) or WM (100 nM), and then incubated with 4-HBA (10 μM) for 18 hr. **P<0.01, ***P<0.001.
