Fig. 5.

Sirt1 contributed 4-HBA to induce formation and maturation of autophagosome and led activation of antiparasitic responses to T. gondii infection. (A) Immunofluorescence micrographs showing the formation of LC3 puncta in BMDMs treated with 4-HBA (10 μM; 18hr) in SC, EX-527 (4 μM), or sirtinol (30 μM). Left panel, representative images. Right panel, LC3 punctate foci in the cells. Scale bar=10 μm. (B, C) BMDMs were infected with GFP-RH strain, followed by treatments of SC, EX-527 (4 μM), or sirtinol (30 μM), and then incubated with 4-HBA (10 μM) for 18 hr. Cells were stained with Alexa 594-conjugated LC3 (red) and DAPI (blue), (B) Immunofluorescence micrographs showing co-localization of GFP-RH strain (green) and LC3 (red). Scale bar=10 μm. (C) Co-locol of LC3 with T. gondii. ****P<0.001. SC, solvent control; Tg, T. gondii.