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. 2019 Dec 23;9(3):e980. doi: 10.1002/mbo3.980

Figure A2.

Figure A2

SDS‐PAGE of Ni‐NTA‐purified enzymes. Lanes 1–4: Paenibacillus sp. 3179 β‐d‐galactosidase at approx. 70 kDa [1:1,200 µg; 2:240 µg; 3:120 µg; 4:24 µg]. Lanes 5–8: Paenibacillus sp. 3179 α‐l‐fucosidase at approx. 50 kDa [5:450 µg; 6:90 µg; 7:45 µg; 8:9 µg]. Lane 9:5 µl PageRuler Unstained Protein Ladder (Thermo Fisher Scientific #26614). Enzyme concentrations were determined by Bradford assay with BSA as standard protein