FIGURE 7.

Effect of hNET mutants on NX binding. Comparison of WT and hNET mutants (A) B_max and (B) K_d for NX determined from saturation binding curves using increasing concentrations of the radiolabeled hNET antagonist [³H]-NX (0.5–50 nM). Non-specific binding was determined in the presence of 200 μM NX. Values are means ± SEM of 3–4 separate experiments for mutants and n = 15 for WT, each performed in triplicate. *Significant mutant effects compared with WT hNET, analyzed by Dunnett’s multiple comparisons test after one-way ANOVA.