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. 2020 Feb 26;5(9):4433–4448. doi: 10.1021/acsomega.9b03523

Figure 13.

Figure 13

Confocal microscopy imaging of (A) DAPI-stained nucleus in the blue channel, (B) Rab11, EEA1, CD63, and LAMP-2 are stained with the corresponding primary and Alexa Fluor 647 conjugate secondary antibody in the far red channel, respectively, and (C) denotes the uptake of coumarin-6-labeled LDC-NPs in differentiated THP-1 cells under the green channel. (D) Merged imaging of all channels with respective bleached pink and white coloration, signifying the colocalization of LDC-NPs in early and recycling endosomal and lysosomal compartments, respectively, at a fixed magnification scale.