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. 2020 Mar 15;388(2):111840. doi: 10.1016/j.yexcr.2020.111840

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — A: Left column D3-3 FISH. The D3-3 metaphase spreads were hybridised with the 17α DNA (green signal) and 21/13α DNA (red signals) probes. The chromosomes were counterstained in DAPI, blue. The HAC (yellow arrow) was labelled by both probes, as visible in the inset. The endogenous chromosome 17 (green arrows) are labelled by 17α only, while the endogenous chromosome 21 and 13 (red arrows) are labelled by the 21/13 probe only. Right column: LWB5 FISH. The LWB5 metaphase spreads were hybridised with the 17α DNA (green signal) and the HPRT DNA (red signal) probes. The chromosomes were counterstained in DAPI, blue. The HAC (yellow arrow) was labelled by both probes, as visible in the inset. The endogenous chromosome 17 (green arrows) were labelled by 17α only, while the endogenous HPRT locus (red arrows) were labelled by the HPRT probe only. B: Clones expressing either GFP or RFP only, or both. Top panels, iPSc DF19.9. Bottom panels, hESc HUES-2. C: Western blot analysis of HPRT expression in the LWB5 clone, and lack of expression in the HT1080 HPRT^−/- parental cell lines compared to HT10180 WT control. GAPDH was used as loading control.