Table 2. Intra-Assay and Interday Assay Precision (% RSD) and Accuracy (% Bias) of the UPLC–MS/MS Method for the Determination of Selegiline in Plasmaa.
| nominal concentration (ng/mL) | observed concentration (ng/mL) | precision RSD (%) | accuracy Bias (%) |
|---|---|---|---|
| Intra-Assay | |||
| 5 | 5.2 ± 0.15 | 2.92 | 4.06 |
| 10 | 11.04 ± 0.63 | 5.68 | 10.37 |
| 50 | 46.64 ± 2.67 | 5.72 | –6.76 |
| 100 | 104.1 ± 2.07 | 1.99 | 4.1 |
| 500 | 510.39 ± 14.3 | 2.80 | 2.08 |
| Inter-Assay | |||
| 5 | 6.01 ± 0.84 | 13.95 | 20.17 |
| 10 | 10.62 ± 0.66 | 6.17 | 6.18 |
| 50 | 47.97 ± 0.88 | 1.84 | –4.05 |
| 100 | 100.3 ± 2.2 | 2.19 | 0.3 |
| 500 | 500.3 ± 1.31 | 0.26 | 0.06 |
a
Data are expressed as the mean ± SD (n = 6). RSD % = [SD/observed concentration (Cobs)] × 100%. Bias % = 100 × [Cobs – nominal concentration (Cnom)]/Cnom.