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Fig. 5. Site-selective cysteine-to-lysine transfer (CLT) with bis-thioester 7: (a) general scheme, (b) LC-MS after transthioesterification of reduced Fab with thioester 7, (c) LC-MS of CLT conjugate 9, (d) LC-MS/MS of the Lys-136 modified peptide; 0 and 1 refer to the number of acyl groups added per species.