Table 2. Melting temperatures (Tm) of self-complementary phosphoroselenolate-modified and native oligodeoxynucleotide duplexes.
| Entry | Sequence 5′→3′ | X = | T m/°C | X = | T m/°C | ΔTm a /°C |
| 1 | d(AXCCCGGGAT) | SedT (ODN 2) | 46.9 | T | 47.6 | –0.7 |
| 2 | d(CXCCCGGGAG) | SedT (ODN 3) | 48.1 | T | 54.3 | –6.2 |
| 3 | d(GXCCCGGGAC) | SedT (ODN 4) | 50.7 | T | 55.6 | –4.9 |
| 4 | d(TXCCCGGGAA) | SedT (ODN 5) | 43.0 | T | 47.0 | –4.0 |
| 5 | d(CGCGAAXTCGCG) | SedT (ODN 6) | 55.9 | T | 57.8 | –1.9 |
aΔTm = Tm(X = SedT) – Tm(X = T). Conditions: ssDNA (10 μM), NaPi (10 mM, pH 7.0), NaCl (100 mM).