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. 2020 Mar 12;18:42. doi: 10.1186/s12964-020-0529-x

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Mitochondrial morphology and function in primary cortical neurons obtained from ncx3+/+ and ncx3−/− mice. (a-left), Imaging mitochondrial morphology in ncx3+/+ and ncx3−/− cortical neurons by confocal microscopy and MitoTracker Red (20 nM), N: neurons; scale bars: 10 μm. (a-right), quantification of the changes in mitochondrial morphology by Image J software. Form Factor (FF) and Aspect ratio (AR) in ncx3−/− neurons. b Confocal analysis of mitochondrial membrane potential and mitochondrial calcium concentration in ncx3+/+ and ncx3−/− cortical neurons. Each bar represents the mean + S.E.M. of the percentage of different experimental values obtained in three independent experimental sessions. *P < 0.05 vs ncx3+/+ and CTL; **P < 0.05 vs OGD