Fig. 3.

Proteasomal inhibition increases transiently overexpressed BACE2 in HEK293 cells. a HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with 0, 10, 15 or 20 μM proteasomal inhibitor MG-132 for 12 h. b Quantification of BACE2 protein levels. c HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with10 μM MG-132 for 0, 6, 12 or 24 h. d Quantification of BACE2 levels. e HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with 0, 5, 10 or 25 μM proteasomal inhibitor ALLN for 12 h. f Quantification of BACE2 protein levels. g HEK293 cells were transfected with pBACE2-mychis. 48 h after transfection, cells were treated with10 μM ALLN for 0, 6, 12 or 24 h. (h) Quantification of BACE2 levels. 9E10 antibody was used to detect myc-tagged BACE2 protein. β-actin served as an internal control. Values represent mean ± SEM; n ≥ 3, *P < 0.05 by one-way ANOVA followed by Tukey’s test