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. 2020 Mar 11;21:66. doi: 10.1186/s12931-020-01333-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of B cell activating factor (BAFF) on the apoptosis of CD4⁺ cells from COPD patients. Peripheral blood mononuclear cells (PBMCs) isolated from COPD patients were treated with recombinant human BAFF (rhBAFF) or rhBAFF plus BR3-Fc (BAFF antagonist) and the apoptosis of CD4⁺ cells was analyzed by flow cytometry. The gating strategy for identifying CD4⁺ cells and CD8⁺ cells is shown (a and b). The representative data of apoptosis of CD4⁺ cells from a COPD patient that were treated with control (c), rhBAFF (d) or rhBAFF plus BR3-Fc (e) are shown. The apoptosis of CD4⁺ cells from COPD patients (n = 13) treated with control, rhBAFF or rhBAFF plus BR3-Fc is shown (f). Data are presented relative to the control which is set at 100%. NS, nonsignificant