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Primer sequences for RT-PCR
| Primers | Accession no. | Sequence (5′–3′) |
|---|---|---|
| PKC-α | ||
| Forward | NM_011101.3 | CCCATTCCAGAAGGAGATGA |
| Reverse | NM_011101.3 | TTCCTGTCAGCAAGCATCAC |
| α-SMA | ||
| Forward | XM_006526606.2 | ACTGCCGAGCGTGAGATTGT |
| Reverse | XM_006526606.2 | TGATGCTGTTATAGGTGGTTTCG |
| TGF-β1 | ||
| Forward | NM_011577.2 | CGAAGCGGACTACTATGCTAAAGAG |
| Reverse | NM_011577.2 | TGGTTTTCTCATAGATGGCGTTG |
| GAPDH | ||
| Forward | XM_017321385.1 | CAGCCTCGTCCCGTAGACA |
| Reverse | XM_017321385.1 | CGCTCCTGGAAGATGGTGAT |
