Fig. 4.

Enforced Expression of CircMRPS35 Suppresses the Growth and Metastasis of Gastric Cancer Cells in vivo. a The image of tumors in nude mice bearing gastric cancer cells. The subcutaneous transplanted model of human gastric cancer was established with SGC7901 cells infected with Lv-circMRPS35 (Lv-Circ) or Lv-control in nude mice (n = 10 in each group). b Tumor volume measured on the indicated days. c Weight of tumor masses. All nude mice were sacrificed on the 21th day after inoculation, and the tumor masses were weighed. d Representative images of circMRPS35 stained by ISH and Ki-67, PCNA stained by IHC. Scale bar, 200 μm. e The image of tumors in nude mice bearing MGC803 cells infected with shCirc-1, shCirc-2 targeting circMRPS35 and shNC lentivirus. f-h Tumor size f, tumor weight g and staining of circMRPS35, Ki-67 and PCNA h in the tumors of the above model in e. i The image of in vivo bioluminescence imaging. Stable SGC7901 cells (infected with Lv-circMRPS35-luci (Lv-Circ) or Lv-control-luci (Lv-Control)) were injected into nude mice (n = 5 in each group) via the tail vein. The luminescence intensity of the mice was measured through in vivo small animal imaging technology 4 weeks later. j Statistical analysis of the luminescence intensity. k H&E staining of representative metastatic lesions in the lungs of nude mice. After the mice were sacrificed, the lungs were divided into eight parts on average, and each part was used for the calculation of metastatic lesion area. Scale bar, 500 μm (left), 200 μm (right). l The total area of metastatic lesions for each mice representing the metastatic tumor volume in the lungs. m The images of in vivo bioluminescence imaging. Stable MGC803 cells (shCirc and shNC infected with luciferase-expressing lentivirus) were injected into nude mice (n = 5 in each group) via the tail vein. The luminescence intensity of the mice was measured through in vivo small animal imaging technology 4 weeks later. n Statistical analysis of the luminescence intensity. o H&E staining of representative metastatic lesions in the lungs of nude mice. p The total area of metastatic lesions for each mice. *P < 0.05, **P < 0.01, ***P < 0.001